ABSTRACT We present an application of self-adaptive supervised learning classifiers derived fromthe Machine Learning paradigm, to the identification of candidate Globular Clus-ters in deep, wide-field, single band HST images. Several methods provided by theDAME (Data Mining & Exploration) web application, were tested and compared onthe NGC1399 HST data described in Paolillo et al. (2011). The best results were ob-tained using a Multi LayerPerceptronwith Quasi Newton learningrule which achieveda classification accuracy of 98.3%, with a completeness of 97.8% and 1.6% contami-nation. An extensive set of experiments revealed that the use of accurate structuralparameters (effective radius, central surface brightness) does improve the final result,but only by ∼5%. It is also shown that the method is capable to retrieve also extremesources (for instance, very extended objects) which are missed by more traditionalapproaches.Key words: Globular clusters; elliptical galaxies; NGC1399; Machine Learning