Combating malware is very important for software/systems security, but to prevent the software/systems from the advanced malware, viz. metamorphic malware is a challenging task, as it changes the structure/code after each infection. Therefore in this paper, we present a novel approach to detect the advanced malware with high accuracy by analyzing the occurrence of opcodes (features) by grouping the executables. These groups are made on the basis of our earlier studies PS-MPC, G2 and NGVCK are within 5 KB. On the basis of obtained promising features, we studied the performance of thirteen classifiers using N-fold cross-validation available in machine learning tool WEKA. Among these thirteen classifiers we studied in-depth top five classifiers (Random forest, LMT, NBT, J48 and FT) and obtain more than 96.28% accuracy for the detection of unknown malware, which is better than the maximum detection accuracy (~95.9%) reported by In these top five classifiers, our approach obtained a detection accuracy of 97.95% by the Random forest.