Face recognition systems have raised concerns due to their vulnerability to different presentation attacks, and system security has become an increasingly critical concern. Although many face anti-spoofing (FAS) methods perform well in intra-dataset scenarios, their generalization remains a challenge. To address this issue, some methods adopt domain adversarial training (DAT) to extract domain-invariant features. Differently, in this paper, we propose a domain adversarial attack (DAA) method by adding perturbations to the input images, which makes them indistinguishable across domains and enables domain alignment. Moreover, since models trained on limited data and types of attacks cannot generalize well to unknown attacks, we propose a dual perceptual and generative knowledge distillation framework for face anti-spoofing that utilizes pre-trained face-related models containing rich face priors. Specifically, we adopt two different face-related models as teachers to transfer knowledge to the target student model. The pre-trained teacher models are not from the task of face anti-spoofing but from perceptual and generative tasks, respectively, which implicitly augment the data. By combining both DAA and dual-teacher knowledge distillation, we develop a dual teacher knowledge distillation with domain alignment framework (DTDA) for face anti-spoofing. The advantage of our proposed method has been verified through extensive ablation studies and comparison with state-of-the-art methods on public datasets across multiple protocols.