METHOD FOR DETERMINING METABOLIC FLUX AFFECTING SUBSTANCE PRODUCTION

Patent №

US 7,809,511

Granted

2010-10-05

Filed 2006

Owner

AJINOMOTO CO., INC.

Lab

AI components

3

kr · planning · hardware

Assignment

Recorded

Dataset

AIPD

2023_r1 edition

Application

11337580

A metabolic flux affecting substance production using cells is determined by 1) creating a stoichiometric matrix based on formulas of biochemical reactions from a substrate through a desired produced substance, 2) selecting the same number of independent metabolic fluxes from all metabolic fluxes as the degree of freedom of the stoichiometric matrix as free fluxes, 3) creating a sufficient number of random combinations of the free fluxes for a statistical analysis and calculating a metabolic flux distribution from each created combination based on the stoichiometric matrix, 4) obtaining a regression equation including a minimum number of free fluxes that shows a correlation with substance production from the calculated metabolic flux distributions by a multivariate statistical analysis, and 5) determining at least one metabolic flux affecting substance production based on a coefficient in the obtained regression equation.

AI classification

AI hardware1.00
Knowledge representation0.85
Planning0.66
Evolutionary computation0.04
Machine learning0.01
Vision0.00
Natural language0.00
Speech0.00

Ownership

AJINOMOTO CO., INC.

assignment · 175360569

Assignors

VAN DIEN, STEPHEN, IWATANI, SHINTARO, USUDA, YOSHIHIRO, MATSUI, KAZUHIKO, UEDA, TAKUJI, TSUJI, YUICHIRO

On an employer assignment, the assignors are typically the inventors.

From the same owner

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