Flow Cytometer Sorter

Disclosed are computer-implemented methods of sorting particles from a particle stream in a flow cytometer. The methods include: calculating sort decision making parameters using the raw event data values from a flow cytometer and a sort logic; performing sort logic computations using the sort logic definition and the sort decision making parameters to generate sort decisions; converting the sort decisions into sort commands; and sending the one or more sort commands to the flow cytometer. Sort logic computations may include algorithmically using conditional branching logic, and may include sort logic equations having mathematical functions characterizing one or more regions of interest in multidimensional data space. Such mathematical functions may be determined based on one or more parameters provided by a user. Also disclosed are corresponding systems having a flow cytometer and a computer.