Method of On-The-Fly Image Stitching

The presently claimed invention provides a method for stitching a plurality of images together in a way with least memory and CPU usage, and minimum file input and output, while still possessing fast computation speed to avoid post-scan delay for whole slide viewing and good stitching quality. The method of the present invention comprises the steps of calculating featureness of each candidate strip of a image by applying a mathematical transformation, calculating offset of the strip with correlation maximum location, calculating stitching reliability of the candidate strip from the pre-defined weighted function of its featureness and the correlation coefficients of each matching block, and determining optimal stitching path with stitching reliability.